Table 1.
Protocols presented in this chapter.
| Assay | Technique Number | Throughput | Specialized Equipment Required* | Result Format |
|---|---|---|---|---|
| Culturing | BP 1 | N/A | N/A | |
| Standard Optical Density | BP 2 | High | Plate Reader | OD Measurement |
| XTT Reduction | AP 1 | High | Plate Reader | OD Measurement |
| Crystal Violet | AP 2 | High | Plate Reader | OD Measurement |
| Inhibition Optical Density | AP 3 | High | Plate Reader | OD Measurement |
| Disruption Optical Density | AP 4 | High | Plate Reader | OD Measurement |
| Dispersal Optical Density | BP 3 | High | Plate Reader | OD Measurement |
| Co-culturing and Analyzing with Standard Optical Density | BP 4 | High | Plate Reader | OD Measurement |
| Colony-Forming Units | AP 5 | Medium | Colonies on Plate | |
| Silicone Square | BP 5 | Medium | Depends on Usage | |
| Cell Adhesion | BP 6 | Medium | Colonies on Plate | |
| Dry Weight | BP 7 | Low | Analytical Balance | Weight of Biofilm |
| Cell Enumeration | BP 8 | Medium | Colonies on Plate | |
| Cell Enumeration Using Florescence Microscopy | AP 6 | Medium | Fluorescent Microscope | Image |
| Pheromone-Stimulated | BP 9 | High | Plate Reader | OD Measurement |
| Microfluidics | BP 10 | Medium | BioFlux EZ1000 | Image / Movie |
| Confocal Imaging | BP 11 | Low | Confocal Microscope | Image |
| Confocal Imaging of Mixed-Species Biofilms | AP 7 | Low | Confocal Microscope | Image |
| Conditioned Media Harvesting | BP 12 | Low | Depends on Usage |
*
Microplate shaking incubators are required for all C. albicans biofilm assays.