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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2018 Jun 7;46(1):e56. doi: 10.1002/cpsc.56

Figure 3.

Figure 3

Cell adhesion to the functionalized alginate beads is enabled by a high aspect ratio rotational bioreactor. a) The bioreactor vessel is a PDMS well cast into a standard 35×10mm petri dish using a custom designed 3D mold (blue). b) The completed bioreactor vessel. c) The bioreactor vessel with 0.25mL of sedimented, functionalized alginate beads. d) Bioreactor vessel after 1hr of incubation with 2 million fetal lung fibroblasts. NOTE: the beads have clumped together indicating successful cellular adhesion. e) Image of the loaded bioreactor vessel in the rotational bioreactor comprised of a lab rotisserie and a custom built bioreactor vessel mount.