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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2018 May 24;46(1):e57. doi: 10.1002/cpsc.57

Fig. 1. Engineered construct for collagen alignment.

Fig. 1

(A) Modify wells in 6-well plates by etching out rectangular sections 2.5 × 1.0 cm in dimension on the bottom with the heated flat end of a spatula; (B) Attach hydrophobic, porous polyethylene pieces (spacers) at the two ends of the rectangular region with vacuum grease; (C and D) Plate the gel mixture onto the spacers before drawing the mixture out onto the rectangular region, allowing the two ends to meet in the middle; (E) Allow the gel to start setting at room temperature for 20 mins and then carefully transfer to the 37°C incubator.