(A) Germline development during early larval stage and adulthood. (B and C) The discrete function of POLA-1, DIV-1, PRI-1, and PRI-2 in GLP-1/Notch-mediated germ cell proliferation either non-cell autonomously or cell autonomously during early larval development and adulthood, respectively. (D) The shared function of POLA-1, DIV-1, PRI-1, and PRI-2 in PUF-mediated GSC maintenance.