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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Muscle Nerve. 2018 Apr 23;58(2):286–292. doi: 10.1002/mus.26135

FIGURE 1. Representative Projections of Entire Muscle Section Image Files.

FIGURE 1

Projections of entire image files of biopsy GNE-M3 are shown to illustrate the fluorescence quantitation process. Biopsy slides were co-stained with membrane marker CAV-3 (secondary antibody AlexaFluor555-labeled) (A) and sialic-acid binding lectin SNA (FITC-labeled) (B). CAV-3/SNA fluorescence overlay image (C). Manual annotation to exclude non-specific areas (green) from analysis (D). Caveolin fluorescence set as a mesh for muscle membrane area (E). SNA fluorescence co-localizing on CAV-3 mesh (F). Fluorescence overlay images of all 9 biopsies are displayed in Supplemental Figure S1.