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Matrix substrate, but not graphene overlay, impacts RGC density. A) Representative fluorescent micrographs of RGCs cultured on glass (top), laminin (middle), or PDL (bottom) with (+) or without (−) graphene overlay. RGCs were labeled with calcein (green) and ethidium homodimer-1 (red). Scale bar = 100 μm. B) Box plot of total cell density (y-axis; cells mm−2) in each culture platform. Asterisks indicate p < 0.05.
