Fig. 1. Thymol inhibits cancer cell proliferation in vitro.
A) MTS cell viability assay of OSCC cell lines, Cal27, SCC4, and SCC9 treated with thymol for 48 h revealed GC50 values of 300 μM, 500 μM, and 550 μM respectively (n=4 per group). B) MTS cell viability assay of a panel of cancer cell lines (HeLa, H460, MDA-231, and PC3) treated with thymol for 48 h revealed GC50 values ranging from 350 μM, to 500 μM (n=4 per group). C) Clonogenic assays of Cal27 and HeLa cells 9 days following a 2 min exposure to thymol (4.3 mM; right panels) or vehicle control (left panels).