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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: FEBS J. 2018 May 24;285(15):2785–2798. doi: 10.1111/febs.14498

Figure 4. Il4rα KO mice show increased c-Caspase 3 after ischemic stroke stimulation in an ex vivo brain slice stroke model.

Figure 4

(A) mRNA levels of Il4, Il4rα, Bcl-2, and Caspase 3 from Il4rα WT and KO were determined by qRT-PCR 12 h and 24 h after 5.5 min OGD. Gapdh was used for normalization. Experiments were performed three times using three to four mice per genotype for each experiment. (B) Western blots were performed to detect both BCL-2 and c-Caspase 3 in explanted brain slices of Il4rα WT and KO 12 h and 24 h after 5.5 min OGD. (C and D) Levels of BCL-2 (C) and c-Caspase 3 (D) protein were determined. GAPDH was used for normalization. Experiments were performed four times using three to four mice per genotype for each experiment. Data represent the mean ± SEM. Statistical analysis, two-way ANOVA followed by Bonferroni’s multiple comparison test (* p<.05; ** p<.01; *** p<.001).