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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: FEBS J. 2018 May 24;285(15):2785–2798. doi: 10.1111/febs.14498

Figure 5. Il4rα KO mice show reduced levels of STAT6 transcript and phosphorylated STAT6 protein after ischemic stroke stimulation in an ex vivo brain slice stroke model.

Figure 5

(A) mRNA levels of Stat6 from Il4rα WT and KO were determined by qRT-PCR 12 h and 24 h after 5.5 min OGD. Gapdh was used for normalization. Experiments were performed three times using three to four mice per genotype for each experiment. (B) Western blots were performed to detect both pSTAT6 and STAT6 in explanted brain slices of Il4rα WT and KO 12 h and 24 h after 5.5 min OGD. (C) Levels of pSTAT6 protein were determined using total STAT6 protein levels for normalization. Experiments were performed three times using three to four mice per genotype for each experiment. Data represent the mean ± SEM. Statistical analysis, two-way ANOVA followed by Bonferroni’s multiple comparison test (* p<.05; ** p<.01; *** p<.001).