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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Dev Dyn. 2018 May 4;247(7):903–913. doi: 10.1002/dvdy.24633

Fig 6. Tbx2 functions as a repressor during germ layer differentiation.

Fig 6

(A) Expression of Tbx2-DBD-EnR inhibits mesendoderm induction by Activin (left panel); expression of Tbx2-DBD-Vp16 does not inhibit Activin-mediated mesendoderm induction (right panel). RT-PCR analysis of animal caps dissected at late blastula stages and cultured until mid-gastrula stages. Tbx2-DBD-EnR or Tbx2 DBD-VP16 RNA (1ng) was injected at early cleavage stages, as indicated. Activin (0.5ng/ml) was added to stage 8 animal caps, as indicated (B) Expression of Tbx2 DBD-VP16 inhibits BMP target gene expression (left panel), and stimulates expression of mesodermal and endodermal marker genes in the absence of exogenous growth factors (left and right panels). RT-PCR analysis of animal caps dissected at late blastula stages and cultured until mid-gastrula stages. Tbx2-DBD-VP16 RNA (1ng) was injected at early cleavage stages, as indicated. The “-RT” lane contains all reagents except reverse transcriptase and was used as a negative control.