Table 1.

Current gating strategies to identify MEPs by flow cytometry and the respective in vitro assays to evaluate their lineage potential.

Species	Name	Immunophenotype	Assay	Lineage Potential	Ref
Mouse	MEP	IL-7Ra-Lin-Sca-1-c-Kit+ FcgRloCD34−	CFC in methylcellulose	20% of Mk/E, 30% of Mk and 10% E and 40% N/A	[2]
Mouse	PreMegE	Lin-cKit+Sca-FcgRII/III-CD41−CD150+CD105−	CFC in methylcellulose	10% of Mk/E, 50% of Mk and 40% E	[16]
Human	MEP	CD34+CD38+IL3RA- CD45RA-	CFC in methylcellulose	10% Mk/E, 5% Mk, 70% E and 15% N/A	[43]
Human	MEP	CD34+CD38+Thy-1−CD45RA−CD135−CD7−CD10−	MS-5 co-culture and CFC in methylcellulose	No colonies in co-culture. E-only colonies in CFC assays	[57]
Human	MEP	CD19-CD34+IL-3Ralphalo/-CD45RA-BAH1+	CFU-Mk/E in megacult and methocult	Less than 3% Mk/E, 3% Mk, 37%E, 55% N/A in megault. E-only in methylcellulose	[42]
Human	MEP (F1)	CD34+CD38+CD10−FLT3CD45RA–CD71-BAH1-	MS-5 co-culture followed by flow cytometry	60% myeloid (GlyA−CD41− CD45+CD11b+), 20% E and 10% Mix (myeloid and E or Mk)	[21]
Human	MEP (F3)	CD34+CD38+CD10−FLT3CD45RA–CD71+BAH1+	MS-5 co-culture followed by flow cytometry	90% E-only	[21]
Human	MEP	CD34+Lin-CD135-CD45Ra-CD38midCD110+CD36-CD41-	Dual CFU-Mk/E in megacult	50% Mk/E, 20% Mk, 25% E in megacult. 90% E and 10%G/M in methylcellulose	[10]