Figure 7.

Transfer of an inflammatory disease by transplantation of Pld3^−/−Pld4^−/− bone marrow. (a) Eight weeks post transfer, whole blood was analyzed for platelet (PLT), red blood cell (RBC), reticulocyte (RET) and white blood cell (WBC) counts from B6.SJL-CD45.1 recipient mice receiving bone marrow of the indicated genotypes: Pld3^−/−Pld4^+/− (dark grey bar), Pld3^+/−Pld4^−/− (light grey bar), Pld3^−/−Pld4^−/− (white bar). Bar indicates mean and symbols indicate individual recipient values. (b) Spleen weight (mg) and splenocyte count after erythrocyte lysis was determined 8 weeks post transfer. (c) Representative flow cytometry plots indicating recipient splenic CD45.2⁺-gated B cell populations stained with B220 and IgM from the indicated bone marrow donors. Summary data shown in d. (d) Numbers of donor (CD45.2⁺) B220⁺IgM⁺ B cells identified in spleens as gated in (c). (e) Numbers of CD45.2⁺TCRβ⁺CD4⁺ (left) or CD8⁺ (right) T cells from the spleens of recipient mice transferred with donor bone marrow of the indicated genotypes. (f) Representative flow cytometry plots of splenic CD45.2⁺ cells from mice receiving bone marrow of the indicated genotypes. Panels show gating and proportions of TCRβ⁺, CD4 and CD8 T cells. Cells gated on either CD4 or CD8 cells were further analyzed by CD62L and CD44 staining. Summary data shown in g. (g) Frequencies and numbers of CD8 T cells staining CD44^hiCD62L^lo (upper panel) or CD44^loCD62L⁺ (lower panel) in the spleens of recipients receiving bone marrow of the indicated genotypes. n=4, 4, 3 for Pld3^−/−Pld4^+/−, Pld3^+/−Pld4^−/−, and Pld3^−/−Pld4^−/−, respectively. Two-tailed T test was used to calculate P values. This experiment was performed once.