Table 1.
Summary of pharmacological data
| Measurement | WT GluN2A | GluN2A-A643D | p value | ||||
|---|---|---|---|---|---|---|---|
| Mean | 95% CI | n | Mean | 95% CI | n | ||
| Glutamate EC50, μM | 5.0 | (4.1, 6.1) | 18 | 1.0 | (0.7, 1.4) | 23 | <0.0001 |
| Glycine, EC50, μM | 1.1 | (0.9, 1.5) | 10 | 0.11 | (0.09, 0.13) | 13 | <0.0001 |
| Mg2+, IC50, μM | 18.6 | (12.9, 27.0) | 13 | 39.4 | (27.5, 56.2) | 12 | 0.004 |
| Proton, % A | 42.8 | (40.5, 45.0) | 8 | 48.0 | (45.1, 50.9) | 13 | 0.010 |
| Zn2+, IC50, nM | 9.1 | (6.6, 12.4) | 12 | 30.3 | (12.7, 72.3) | 11 | 0.007 |
| Zn2+, Residual Current (%)B | 30.9 | (24.3, 37.4) | 12 | 67.3 | (60.7, 73.9) | 11 | <0.0001 |
A
% current remaining measured at pH 6.8 compared to pH 7.6 at maximal L-glutamate and glycine activation
B
current remaining in maximum Zn2+ concentration (expressed as % of maximal receptor activation in the absence of Zn2+)
p value determined by unpaired t-tests (GraphPad Prism 5.0) comparing WT vs. mutant receptors. For EC50 and IC50 measurements log values were used for statistical comparison.
n = the number of oocytes evaluated for the measured endpoint.
CI = confidence interval