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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Genesis. 2018 Apr 19;56(6-7):e23108. doi: 10.1002/dvg.23108

Figure 1. Daam1 is required for premigratory NC development.

Figure 1

Eight-to-sixteen-cell embryos were unilaterally injected into animal blastomeres with Daam1 MO (20 ng), control MO (CoMO, 20 ng) or N-Daam1 RNA (10-20 pg) as indicated. A-C, E-G, In situ hybridization of stage 14/16 embryos with different antisense RNA probes. The arrows (B, F, G) point to the injected side with inhibited NC marker. Light blue is the lineage tracer (nĪ²Gal). Anterior views are shown. NC territory did not change in wild-type (not shown) and CoMO-injected embryos (A, E). D, Daam1 domain structure and interactions are shown. H, Frequencies of embryos with altered NC marker expression. The graphs indicate the percentage of embryos with decreased gene expression. The number of embryos analyzed is shown on top of each bar.