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. 2018 Aug 22;8:12590. doi: 10.1038/s41598-018-30820-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The insulin signaling pathway and GluT4 membrane translocation were monitored. (A) Insulin signaling pathway changes were assayed in cultured 3T3-L1 cells. (B) Semi-quantification of (A) based on Image J. (C) GluT4 membrane translocation was monitored by immunofluorescence. (D) Expressions of GluT4 in membrane and cytoplasmic proteins were assayed by western blot. (E) The semi-quantification of (C) using Image J.