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. 2018 Aug 22;8:12560. doi: 10.1038/s41598-018-29685-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structure-based high-throughput screening to target SERCA2a-PLB complex. (a) Schematic diagram illustrating expression vectors and structural model of GFP-SERCA2a free or bound to RFP-PLB. (b) Time-resolved fluorescence waveforms as a readout to measure FRET between GFP-SERCA2a and RFP-PLB. FRET is calculated as the change between lifetimes (exponential decay times) of donor-only and donor-acceptor samples (τ_D and τ_DA).