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. 2018 Aug 22;8:12560. doi: 10.1038/s41598-018-29685-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Ca and Tg on SERCA2a-PLB biosensor. (a) Left- FLT measurements in saponin-permeabilized cells under conditions of low Ca (100 nM) and high Ca (10 µM), and in the absence and presence (100 nM) of the SERCA inhibitor thapsigargin (Tg). Right- FRET readout calculated from (a) shows a Ca-dependent decrease in FRET, that is ablated by addition of Tg. Error bars indicate SD (n = 3). Statistical differences determined using 1-way ANOVA followed by Tukey post hoc analysis (more detailed analysis is provided in Supplemental Table 4). *P ≤ 0.01 and N.S. indicating P ≥ 0.05. (b) Distance distributions between donor on SERCA and acceptor on PLB, based on Eq. (10).