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. 2018 Aug 22;8:12569. doi: 10.1038/s41598-018-30043-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of GmPIF4a-d,f,g transcription factors under different photoperiods and temperatures. (A–F) The expression levels of mRNA transcripts tested at different time points (0, 4, 8, 12, 16, 20 and 24 h) under long day photoperiod conditions. (G–L) The expression levels of mRNA transcripts tested at different time points (0, 4, 8, 12, 16, 20 and 24 h) under SD photoperiod conditions. (M–R) The expression levels of mRNA transcripts tested at three different temperatures (25 °C, 30 °C, and 35 °C) under both long day and SD photoperiods. Data are from three independent biological experiments, and error bars indicate standard deviations. Significant differences between data were calculated using Student’s t-test (all time points have been compared to time point of 0 h). Significant differences are indicated with asterisks (*), P < 0.05. Intervals on X-axis represent time points/temperature points and intervals on Y-axis represent expression levels calculated by the 40-ΔCt method. The shaded area of the graph represents night. At 0 h, samples were collected in the dark, just before the day began (dawn), and for temperature experiments all samples were collected just before the day began (dawn).