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. 2018 Aug 22;8:12569. doi: 10.1038/s41598-018-30043-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression analysis of GmPIF4b in Col-0 Arabidopsis background. (A) Binary vector construct containing GmPIFb CDS driven by the 35s promoter. (B) Early flowering phenotype in GmPIF4b-ox lines at SD 25 °C, (C) Hypocotyl length phenotype in GmPIF4b-ox lines at SD 25 °C. (D) Measurement of hypocotyl length of different lines. (E) Mean days to flowering of different lines. (F) Expression levels of GmPIF4b in WT and GmPIF4b-ox lines normalized against Arabidopsis actin gene. The significant differences between data were calculated using Student’s t-test. Significant differences are indicated with asterisks: (*)P < 0.05 and (**)P < 0.01. Error bars represent SD, n = 10. WT plants appear orange because they were observed in blue light.