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. 2018 Aug 22;8:12574. doi: 10.1038/s41598-018-30109-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HCMV-DB infects HMECs. (A) HCMV cytopathic effect (CPE) in MRC5 cultures infected with supernatants harvested from HCMV-DB infected HMECs. Heat inactivated HCMV-DB is used as a negative control. (B) Clusters of round cells in HMECs infected with HSV-1. No CPE detected with the direct infection of HMECs with EBV and VZV. (C) Western blotting showing the time-course expression of pUS28 in HCMV-DB infected HMECs.