Fig. 5.

Innate responses in the blood recruit viral targets to tissues and promote viral persistence. a Early cellular innate immune responses in PTM with and without detectable ZIKV RNA in the rectum at day 7. Each panel shows the AUC of pDCs measured by flow cytometry in the blood between days −10 to +4 or the concentration of plasma MCP-1 measured at 2 dpi in animals with or without detectable ZIKV in the rectum at 7 dpi. Lines indicate the median. Differences between animals with no virus or virus in the rectum were evaluated using Wilcoxon tests. Unadjusted p-values are shown. b Correlations between viral burden in the PLN (AUC, days −10 to +21) and either pDC frequency (AUC, days −10 to +4) in the blood (left panel) or the concentration of plasma MCP-1 measured at 2 dpi (right panel). c Scatter plot and Spearman's correlation analysis of the relationship between the concentrations of plasma MCP-1 measured at 2 dpi and mDCs (AUC, days −10 to +21) and non-classical monocytes (AUC, days −10 to +21) in the PLN. Significance of the unadjusted Spearman's correlation test p-values are indicated as follows: *p ≤ 0.05, **p ≤ 0.01