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. 2018 Aug 22;8:12628. doi: 10.1038/s41598-018-31131-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcript levels of maize betaine aldehyde dehydrogenase (BADH) genes BADH1 (A) and BADH2 (B) in maize leaves in response to treatment with L-NAME. Expression levels are relative to the average level of expression of three reference genes (Elongation Factor 1α, β-tubulin and Actin 2) in each treatment. For both (A,B), DETA/NO was used to offset the effect of L-NAME on nitric oxide content. Error bars represent means ± S.D. (n = 3). Different letters above bars indicate statistically significant differences between means (p ≤ 0.05).