Fig. 5.

Two immunizations with nucleoside-modified A/California/07/2009 HA mRNA-LNP vaccine elicits protection from A/California/07/2009 and Puerto Rico/8/1934 viruses. Mice received two i.d. (3, 10, or 30 µg) or i.m. (10, 30, or 90 µg) immunizations of A/California/07/2009 HA mRNA-LNPs or 30 (i.d.) or 90 (i.m.) µg of poly(C) RNA-LNPs at week 0 (prime) and 4 (boost). Animals were challenged with lethal doses of homologous A/California/07/2009 (a, b) or heterologous A/Puerto Rico/8/1934 viruses (c, d) 5 weeks after the second immunization and weight loss and survival were followed. Two independent experiments were performed. n = 5 mice and each weight loss line represents one animal