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. 2018 Aug 22;8:12599. doi: 10.1038/s41598-018-30908-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thirty minutes incubation of 5-ALA induced increased ROS generation in a dose-dependent manner. (A) SCC-13 and (B) A431 cells incubated with 5-ALA at 36° for thirty minutes followed by 1000 seconds of blue lights induced a dose-dependent increase in ROS generation. Error bars represent mean ± standard error of the mean; *P < 0.05 compared to 0 mM control.