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. 2018 Aug 22;8:12599. doi: 10.1038/s41598-018-30908-6

Figure 3.

Figure 3

Thermal 5-ALA incubation induced apoptosis in SCC-13 and A431 cells. (A) 5-ALA incubated at 21 °C to 42 °C for thirty minutes followed by 1000 seconds of blue light induced a temperature-dependent increase in apoptosis in SCC-13 and (B) A431 cells. Bars represent average percent annexin-v positive cells in each treatment and control group. Error bars represent the mean ± standard error of the mean. *P < 0.05. (C) Representative annexin-V vs. 7-AAD flow cytometry plots for 21, 24, 27, 30, 33, 36, 39, 42 °C thermal incubation. Q1 – early apoptotic cells, Q2 – late apoptotic/necrotic cells, Q3 – necrotic cells, Q4 – viable cells. Apoptotic cells populations are highlighted in red. Experiments were performed in triplicate.