Table 4.

ROS generation of 5-ALA and control samples incubated at variable temperatures.

ROS (MFI)
Temperature	21 °C	24 °C	27 °C	30 °C	33 °C	36 °C	39 °C	42 °C
SSC-13
5-ALA	9833.33 ± 103.19	9909.67 ± 163.98	9899.00 ± 266.70	10,349.67 ± 81.43*	10,335.67 ± 152.43*	11,578.67 ± 110.92*	13,034.33 ± 96.06*	12,156.67 ± 64.09*
Control	9301.67 ± 73.09	9449.33 ± 85.53	9205.00 ± 255.34	9192.00 ± 119.46	9355.67 ± 226.82	9565.67 ± 296.33	10,025.33 ± 253.61	9343.33 ± 63.83
A431
5-ALA	4522.33 ± 55.41	5332.00 ± 24.70	5466.00 ± 39.00*	5726.00 ± 58.23*	5710.33 ± 96.20*	6242.67 ± 124.71*	6099.33 ± 81.49*	6593.00 ± 48.68*
Control	4521.67 ± 32.77	5169.33 ± 139.26	5105.67 ± 98.20	4980.33 ± 59.48	4962.00 ± 21.13	5522.33 ± 25.67	5238.67 ± 35.67	5340.33 ± 143.87

Abbreviations: 5-ALA – 5-aminolevulinic acid, ROS – Reactive Oxygen Species, MFI – Median Fluorescent Intensity. P < 0.05 denoted by asterisk (*).