Table 2.
Kinetic parameters of HIF-2α peptides binding to purified VBC complex
| VBC complex | |||
|---|---|---|---|
| Peptide | Kd (nM) | ka (x104) (Ms−1) | kd (x10−3) (s−1) |
| HIF-2αOH (wild-type) | 196 ± 17 | 4.1 ± 0.4 | 7.8 ± 0.2 |
| HIF-2α (wild-type) | No binding | ||
| HIF-2αOH (L529P) | 558 ± 31 | 4.0 ± 0.4 | 22 ± 3 |
| HIF-2αOH (A530T) | 513 ± 21 | 3.4 ± 0.3 | 18 ± 2 |
| HIF-2αOH (A530V) | 219 ± 15 | 2.7 ± 0.2 | 5.8 ± 0.2 |
| HIF-2α (P531A) | No binding | ||
| HIF-2αOH (Y532C) | 998 ± 200 | 4.9 ± 0.2 | 50 ± 10 |
| HIF-2αOH (M535I) | 216 ± 9 | 3.1 ± 0.2 | 6.7 ± 0.2 |
| HIF-2αOH (G537R) | 348 ± 13 | 3.0 ± 0.2 | 10 ± 0.3 |
| HIF-2αOH (D539E) | 253 ± 12 | 3.6 ± 0.05 | 9.0 ± 0.1 |
| HIF-2αOH (F540L) | 345 ± 29 | 3.1 ± 0.3 | 10 ± 0.6 |
Values represent mean of three experiments conducted with independently purified protein ± SEM
Kd: affinity constant, ka: association rate, kd: dissociation rate