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. 2018 Aug 22;9:3369. doi: 10.1038/s41467-018-05548-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — JMJD3 regulates dynamic H3K4me3 and H3K27me3 modifications in the promoter of myelopoietic genes. a Box plots showing the differences of H3K4me3 (left) and H3K27me3 (right) occupancies around the promoter (with 3 kb upstream or downstream of the TSS) of parental and JMJD3 knockout HL-60 cells. b The loci of the downregulated genes in JMJD3 deficiency exhibit a decrease in H3K4me3 (blue dots, left panel) but an increase in H3K27me3 level (red dots, right panel). FPKM, fragments per kilobase of transcript per million fragments mapped. c Genome browser tracks representing the binding sites of H3K4me3 and H3K27me3 at the C/EBPβ or RIPK3 gene locus in parental and JMJD3 knockout HL-60 cells. d, e ChIP–qPCR assay for H3K4me3 (d) and H3K27me3 (e) at the C/EBPβ, RIPK3 or IFNB gene locus in parental and JMJD3 knockout HL-60 cells. f ChIP–qPCR assay for JMJD3 occupancy at a number of gene loci in parental and JMJD3 knockout HL-60 cells. g, h ChIP–qPCR assay for H3K4me3 (g) and H3K27me3 (h) at the C/EBPβ or RIPK3 gene locus in HL-60 cells transduced with empty vector or JMJD3-expressing vector. Data are shown as the mean ± SEM; *p < 0.05, **p < 0.01