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. 2018 Aug 22;9:3369. doi: 10.1038/s41467-018-05548-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — JMJD3 upregulates and partners with C/EBPβ to regulate the expression of key myelopoietic genes in AML cells. a GSEA of the expressing profile of HL-60 cells transduced with control or JMJD3-expressing vector using a C/EBPβ target genes-associated signature. b Venn diagram showing the overlap between C/EBPβ promoter targets in C/EBPβ-overexpressed HL-60 cells (red, see also Supplementary Data 4) and the upregulated genes in JMJD3-overexpressed HL-60 cells (gray, see also Supplementary Data 2) with a p value calculated by hypergeometric test. c GFP⁺ murine AML BM cell samples from PML-RARα- (upper panel), AML1-ETO9a- (middle panel), or MLL-AF9- (low panel) expressing transgenic mice were transduced with an empty vector or JMJD3-expressing vector, and the mRNA levels of a number of genes were measured by qRT-PCR. d Genome browser tracks representing the binding sites of C/EBPβ at the C/EBPβ, JUNB, JMJD3 or IFNB1 gene locus. e ChIP–qPCR assay for C/EBPβ or IgG occupancy at the C/EBPβ, JUNB, JMJD3 or IFNB1 gene locus in C/EBPβ-overexpressed HL-60 cells. f ChIP–qPCR assay for JMJD3 occupancy at the C/EBPβ, JUNB, JMJD3 or IFNB1 gene locus for NC or C/EBPβ knockdown HL-60 cells transduced with control or JMJD3-overexpressed vector. g qRT-PCR assay on the mRNA levels of a number of genes when HL-60 cells were transduced with control or C/EBPβ-expressing vector. h qRT-PCR assay on the mRNA levels of a number of genes when JMJD3-overexpressed HL-60 cells were transduced with or without C/EBPβ siRNA. i qRT-PCR assay on the mRNA levels of a number of genes when JMJD3-overexpressed HL-60 cells were transduced with or without RIPK3 siRNA. j–l The number and typical pictures of the colonies for NC or C/EBPβ knockdown HL-60 cells that had been transduced with control or JMJD3-overexpressing vector. m 32D cell lysates were immunoprecipitated with Jmjd3 antibody and subsequently subjected to western blot with Jmjd3, C/ebpα or C/ebpβ antibody: IgG served as negative IP control. n Heatmap representation of C/ebpβ and Jmjd3 tag density centered on C/ebpβ ChIP-seq peaks in 32D cells. Data are shown as the mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001