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. 2018 Aug 22;8:12533. doi: 10.1038/s41598-018-30319-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Haemagglutinating and neuraminidase activity of bat paramyxovirus B16-40. (A) Haemadsorption activity was determined with mock-infected and bat paramyxovirus B16-40-infected MARC-145 cells with 0.5% chicken RBCs. The bat paramyxovirus B16-40-infected cells showing cytopathic effects were surrounded by chicken RBCs. (B) Neuraminidase activity of the bat paramyxovirus B16-40 isolated in this study was determined by using the NA-Fluor assay. NA activity, which corresponds to the production of product (4-MU), was determined by comparing the average relative fluorescence units (RFU) from the non-virus control and the virus sample.