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. 2018 Aug 22;9:3373. doi: 10.1038/s41467-018-05619-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Structural basis of the formation of the MRJP1 oligomer. a The surface potential (±1 kBT/e) of the MRJP1 oligomer. The surface coloured as a gradient ranging from red (negative) to blue (positive). Details of the interfaces between apisimin and the neighbouring symmetry-generated apisimin molecule are shown in b. Two apisimin protomers are related by a twofold rotation axis, perpendicular to the plane of the page. c Analytical ultracentrifugation of the MRJP1 oligomer at pH 8. The c(S) distribution from sedimentation velocity analysis is shown. d Gel filtration of the MRJP1 oligomer at different pH values