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Competitive EMSA. The purified TetR-His protein binds to the norM promoter from strain FA19 in a specific manner. Lane 1, hot probe N11/N14* alone; lane 2, hot probe N11/N14* plus 8 μg of TetR-His; lanes 3 to 5, hot probe N11/N14* plus 8 μg of TetR-His plus 100×, 200×, and 400×, respectively, unlabeled N11/N14; lanes 6 to 8, hot probe N11/N14* plus 8 μg of TetR-His plus 100×, 200×, and 400×, respectively, unlabeled rnpB.
