Incorporation of radioactive isotopes in the E. coli BW25113 and BW25113 ΔsbmA strains. (A) Incorporation of radioactive leucine in an E. coli BW25113 culture in the absence (no peptide [No pept.]) or the presence of 1 μM Bac5(1-15), Bac5(1-25), or Bac5(1-31). (B to D) Incorporation of radioactive uridine (B), thymidine (C), and leucine (D) in an E. coli BW25113 culture in the absence (no peptide) or the presence of 1 μM Bac5(1-25) or erythromycin (Ery). (D) The incorporation of radioactive leucine in an E. coli BW25113 ΔsbmA culture was also determined in the absence (no peptide) or the presence of 1 μM Bac5(1-25). Error bars represent the standard deviation from the mean from three independent experiments. WT, wild type.