FIG 4.

Toe-printing analysis to monitor the effect of Bac5 fragments on translation. Fluorescence scan of a polyacrylamide gel of toe-print samples obtained in the absence or presence of increasing concentrations of the Bac5(1-15), Bac5(1-25), and Bac5(1-31) peptides. Water instead of antibiotics or peptide was added to a reaction mixture as a control (lane −). Thiostrepton (ThS) was chosen as a control for the inhibition of elongation, and edeine (Ede) was chosen as a control for the inhibition of initiation. ThS stalls ribosomes at the AUG site, and Ede prevents the formation of the initiation complex. PPP, the position where ribosomes stall on the proline triplet motif; FL, the position of the reverse transcription band corresponding to the full-length mRNA; lanes C, U, A, and G, the DNA sequencing lanes of the template.