FIG 5.

Toe-printing assay on Bac5 fragments and Bac7 alone and in the presence of erythromycin. A fluorescence scan of a polyacrylamide gel of toe-print samples obtained in the absence or presence of increasing concentrations of peptides. Water only instead of antibiotics or peptide was added to a reaction mixture as a control (lane −). The already characterized Bac7(1-35) peptide was used for comparison. All the peptides were used at the identical concentration of 20 μM. Samples in the right panel were pretreated with the peptides and then exposed to 5 μM erythromycin. The positions of the AUG, Asp, and Thr sites are indicated by arrowheads. Lanes C, U, A, and G, the DNA sequencing lanes of the template; FL, full-length RNA.