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. 2018 Jul 27;62(8):e00283-18. doi: 10.1128/AAC.00283-18

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Copyright © 2018 Pryjma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Effect of rifabutin and clarithromycin combinations on M. abscessus viability. The following antibiotics were added to M. abscessus ATCC 19977 cultures: clarithromycin at 1× (0.4 mg/liter), 1/2×, or 1/4× MIC (A); rifabutin at 1× (6.3 mg/liter), 1/2×, or 1/4× MIC (B); or a combination of clarithromycin and rifabutin at 1×, 1/2×, or 1/4× MIC of each antibiotic (C). The effect of rifabutin at higher clarithromycin concentrations was tested by incubating M. abscessus with rifabutin alone at 1× to 1/16× MIC (D) or in combination with 50× MIC (20 mg/liter) of clarithromycin (E). CFU were determined at 24-h intervals after antibiotic addition. Data points are the means from three replicates with standard deviations presented as error bars.