TABLE 2.
Cell-dependent activity of GS2 against HCV replicons
| HCV replicon systema and genotype | EC50a (nM) |
Fold (GS2/GS-6620) | |
|---|---|---|---|
| GS-6620 | GS2 | ||
| Stably transfected Huh-Lunet cellsb | |||
| 1a | 110 ± 50 | 380 ± 150 | 3.5 |
| 1b | 300 ± 140 | 2,200 ± 1,300 | 7.2 |
| 2a | 500 ± 160 | 25,000 ± 11,000 | 50 |
| Transiently transfected Huh-1C cells | |||
| 1a | 41 ± 19 | 140 ± 60 | 3.5 |
| 1b | 35 ± 15 | 110 ± 20 | 3.1 |
| 2a | 27 ± 10 | 61 ± 8 | 2.3 |
a
All cells were treated for 3 days. EC50s represent averages ± standard deviations from >3 independent experiments.
b
The stable replicon cell lines were individual clones selected from transfected Huh-Lunet cells.