FIG 2.
Time-kill curves. Rifabutin (RFB) was tested alone or in combination with imipenem (IPM) and the β-lactamase inhibitor avibactam (AVI) against M. abscessus CIP104536 and its derivative ΔblaMAb. (a) Time-kill curves of rifabutin at 16 μg/ml alone or in combination with imipenem (8 and 32 μg/ml) against M. abscessus CIP104536. (b) Time-kill curves of rifabutin at 16 μg/ml alone or in combination with imipenem (8 and 32 μg/ml) against the ΔblaMAb derivative. (c) Time-kill curves of rifabutin at 1 and 8 μg/ml alone or in combination with imipenem (8 μg/ml) with or without avibactam (4 μg/ml) against M. abscessus CIP104536. The number of CFU was determined after 0, 48, and 72 h of exposure to antibiotics. Values are means of three independent experiments. Bars represent the standard deviations.