FIG 1.

Few T cells in granulomas of M. tuberculosis-infected macaques coexpress inhibitory receptors. (A) Frequency of CD3⁺ cells expressing inhibitory receptors PD-1, CTLA-4, or LAG-3 in individual granulomas from M. tuberculosis-infected cynomolgus or rhesus macaques. (B) CD4 T cells express inhibitory receptors more frequently than CD8 T cells. P values indicated on the figure were determined by a Wilcoxon matched-pairs signed-rank test. (C) Frequency of coexpression of inhibitory receptors in granulomas. Each point indicates a granuloma, each color corresponds to an NHP (12 NHPs), circles indicate cynomolgus macaques, and squares indicate rhesus macaques. (D) Clusters of granulomas and TB pneumonia samples have similarly low frequencies of CTLA-4 and LAG3 and similarly high frequencies of PD-1 alone. There is low coexpression of inhibitory receptors in clusters of granulomas. Each point indicates a cluster, consolidation, or pneumonia lung sample, each color corresponds to an NHP (6 NHPs), circles indicate cynomolgus macaques, squares indicate rhesus macaques, and the diamond indicates a TB pneumonia sample. Horizontal lines indicate medians.