TABLE 2.

Example analysis of in silico versus in vivo T cell exhaustion data^a

^aTable 2.3 displays the direct comparison between exhaustion in our matching simulations and the level of observed exhaustion in NHP samples. Tables 2.1 and 2.2 explain our method of matching and aggregating data. Table 2.1 displays 1 of the 44 NHP granuloma samples that we used for calibration. The right-most column shows the number of simulations that matched the NHP sample CFU values and CD3 count. Each simulation could match the criteria across several time points. Table 2.2 shows each of the five simulations that calibrated to NHP sample 13516_RLL 17_20 cluster and the average percentage of exhausted T cells across the simulation time points that matched NHP sample criteria. Finally, we aggregated the data to display an overall sense of model fit by selecting the “median of the means” for each matching simulation. Thus, for NHP sample 13516_RLL 17_20 cluster, the median percentage of exhaustion across all samples that matched was 0.0%. Flow cytometry data shows that this sample had 0.66% of T cells that showed two or more exhaustion markers.