FIG 14.

Immunoblot analysis of metabolic and virulence-associated proteins of 8S strain with increased levels of three SCFAs. 8S strain was propagated in BSKII plus 6% normal rabbit serum supplemented with 0 (lanes 1, 5, and 8), 30 (lanes 2, 6, and 9), 60 (lanes 3, 7, and 10), and 90 (lane 4) mM sodium acetate, sodium propionate, or sodium butyrate as indicated above the SDS-PAGE gel. (A) Total stain of protein lysates separated using SDS–12.5% polyacrylamide gel and stained with Coomassie blue. Note the presence of increased levels of OspC at 90 mM (lane 3) concentration of each of the SCFAs. (B) Immunoblot analysis using antisera specific to a variety of borrelial determinants that play key roles in the metabolism and pathogenesis of B. burgdorferi (indicated to the right of each blot). Molecular masses, in kDa, are indicated to the left.