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. 2018 Aug 22;86(9):e00217-18. doi: 10.1128/IAI.00217-18

FIG 5.

FIG 5

Transcriptional levels of select ORFs in response to supplementation with propionate. Real-time reverse transcription-PCR to detect levels of rpoS, flaB, and ospC in different borrelial strains is shown. Total RNA was extracted from wt, csrABb mutant, ct, 8S, and 7D strains propagated at pH 7.6 and 32°C in BSKII medium supplemented with 0, 30, 60, or 90 mM sodium propionate and converted to cDNA, followed by quantitative real-time PCR using primers specific to rpoS, flaB, and ospC as described in Materials and Methods. The values for all samples were normalized relative to the values for recA, and the change in the CT values for each transcript was obtained as an average of the values determined for each sample analyzed in triplicate. The 2−ΔΔCT values for each transcript from various strains are shown as fold difference on the y axis with the corresponding error bars. The ΔCT values obtained for all strains were subjected to an unpaired Student t test implemented in Prism software. The asterisks indicate levels of significance: ***, P < 0.001; **, P < 0.01; *, P < 0.05.