FIG 9.

Immunoblot analysis of metabolic and virulence-associated proteins of B. burgdorferi strain B31-A3 (wt) with increased levels of butyrate. B. burgdorferi was propagated in BSKII plus 6% normal rabbit serum supplemented with 0 (lane 1), 30 (lane 2), and 90 mM (lane 3) butyrate. (A) Total stain of protein lysates separated using SDS–12.5% polyacrylamide gel and stained with Coomassie blue. Note the presence of increased levels of OspC at 90 mM concentration of acetate (lane 3). (B to E) Immunoblot analysis using antisera specific to borrelial regulatory proteins (B), lipoproteins and oligopeptide permease (C), enzymes (D), and proteins (E) serving as loading controls. Molecular weights, in kDa, are indicated to the left.