FIG 3.

IRAK1 and p38 MAP kinase signaling regulates IL-36γ-inducible PGLYRP2 and hBD2 expression in oral epithelial cells. (A and B) TIGK cells were transfected with an IRAK1 (+) or control (−) siRNA and then subsequently stimulated with IL-36γ (100 ng/ml) for 2 h. PGLYRP2 (A) and hBD2 (B) mRNA levels were then measured (n = 3). (C to F) TIGK cells were treated with 5 μM SB203580 or 10 μM U0126 (C and D) or 10 μM BAY11-7082 (E and F) for 30 min and then stimulated with IL-36γ for 2 h. PGLYRP2 (C and E) and hBD2 (D and F) mRNA levels were then measured (n = 3). ***, P < 0.001; **, P < 0.01; *, P < 0.05.