TABLE 1.
Bacterial strains, plasmids, and primers
| Strain, plasmid, or primer | Description or sequence | Source or reference |
|---|---|---|
| Strains | ||
| PAO1 | Wild type | R. E. Hancock |
| eddB::lux | Mini-Tn5-lux insertion mutant in PA3909 (EddB); transcriptional lux fusion | 29 |
| eddA::lacZ | Mini-Tn5-lacZ insertion mutant in PA3910 (EddA) | 46 |
| ΔeddAB | Unmarked deletion mutant of eddAB | This study |
| xcpU::lux | Mini-Tn5-lux insertion mutant in xcpU; nontranscriptional lux fusion | 45 |
| Plasmids | ||
| pEddA | EddA as an EcoRI-HindIII fragment in pPSV37 with a C-terminal His tag (EddA-His6) | This study |
| pEddB | EddB cloned as a BamHI-XhoI fragment into pUCP22 | This study |
| pPSV37 | IPTG-inducible expression vector; Genr | 44 |
| pUCP22 | Multicopy shuttle cloning vector; Ampr Genr | 47 |
| Primers | ||
| 16SrtF | GAAATCCCCGGGCTCAACCTG | 45 |
| 16SrtR | CCCCACGCTTTCGCACCTCA | 45 |
| EddB-F | GAGGATCCATGCACCCCTTGCGTAACGC | This study |
| EddB-R | GACTCGAGCTACCTGCGGTGCTTCTTCATCG | This study |
| EddA-soe-F | AAGCCTGAAACTCACTGAAG | This study |
| EddA-soe-R | ATACGCATGCCGCCGATATCCGGATTGATCCCGAAAC | This study |
| EddB-soe-F | CGGCGGCATGCGTATAGGAAAGGGCTATTCCTACG | This study |
| EddB-soe-R | TGGAGTGACCTCCGTTCC | This study |
| EddA-RBS-F | ATGCGAATTCAGGAGGAAACGATGAGTGGGATGGACCTCAAGCGC | This study |
| EddA-6×His-R | ATGCAAGCTTTCAGTGGTGATGGTGATGATGGGCGCCGTCGGGCTGCAGTTCCTG | This study |