FIG 3.

OhrR binds specifically to ohr and ohrR promoter DNA. (A) Electropherogram traces of DNase I-digested ohr promoter DNA. (B) Electropherogram traces of DNase I-digested ohrR promoter DNA. Red, DNA only; blue, DNA incubated with OhrR at a stoichiometric DNA/protein ratio of 1:4. The protected regions are expanded in the lower panels, with the sequence being identified at the bottom. Numbering is relative to the translational start, defined as position +1. For ohrR promoter DNA, the ATG codon is underlined. For ohr promoter DNA, a sequence resembling the published OhrR consensus sequence is identified by a dashed underline; the asterisk marks a hypersensitive site. The results are representative of those from at least three replicates.