FIG 5.

OhrR represses ohr expression. (A) Expression of ohr in WT and WTe (the WT strain containing the empty plasmid) cells and in ΔohrR and ΔohrRc (the ΔohrR strain complemented with ohrR) cells. The hatched green bar confirms abundant ohr expression in Δohr cells complemented with ohr (Δohrc). (B) Expression of ohrR in WT and WTe cells and in Δohr and Δohrc (the Δohr strain complemented with ohr) cells. The hatched orange bar confirms abundant ohrR expression in ΔohrR cells complemented with ohrR. Transcript levels are reported relative to the level of transcription of the reference gene (glutamate synthase) and were determined using the 2^−ΔCT method. Error bars represent standard errors from three independent experiments. Asterisks directly above the bars denote statistically significant differences in expression compared to that in WT cells, as determined by an unpaired Student t test (**, P < 0.001).