FIG 3.

Identification of epitopes predicted from the total M. tuberculosis ribosomal proteome. Mice (C57BL/6) (n = 3) were immunized with 5 × 10⁷ CFU IKEPLUS i.v. Two weeks later, CD4⁺ T cells were purified from splenocytes and tested for IFN-γ production by an EPLISPOT assay in response to ex vivo stimulation with 153 mycobacterial ribosomal peptides, as indicated (10 μg/ml). Responses that are significantly different from those under the control DMSO conditions are indicated (*, P < 0.05). Positive-control wells were stimulated with concanavalin A (ConA) (5 μg/ml). Data shown represent mean values and standard errors for quadruplicate samples and are representative of results from two independent experiments.