TABLE 1.

Immunogenic epitopes identified by peptide screens of mycobacterial ribosomal proteins^a

Peptide	Residues	Sequence	P value
Rv0652 (RplL)
7	93–107	AKDLVDGAPKPLLEK	0.0022
127	91–105	KEAKDLVDSAPKPLL	0.0001
Rv2904 (RplS)
10	69–83	VERTFPVHSPNIDHI	0.0001
11	15–29	DIPAFNPGDTINVHV	0.0290
12	65–79	YGVGVERTFPVHSPN	0.0240
13	73–87	FPVHSPNIDHIEVVT	0.0002
Rv0707 (RpsC)
16	210–224	GKRELAAAAPAGADR	0.0240
20	206–220	DIVGGKRELAAAAPA	0.0167
32	182–196	IDYGLYEAKTTFGRI	0.0240
Rv0640 (RpIK)
43	12–26	KLQIVAGQANPAPPV	0.0025
44	67–81	SFTFTLKTPPAAKLL	0.0290
45	16–30	VAGQANPAPPVGPAL	0.0167
Rv0651 (RpIJ)
123	146–160	KAAGLFNAPASQVAR	0.0001
124	151–165	FNAPASQVARLAAAL	0.0001
Rv0683 (RpsG)
56	16–30	PVYGSQLVTQLVNKV	0.0188
Rv0700 (RpsJ)
65	32–46	TGASVVGPVPLPTEK	0.0006
67	28–42	TVVRTGASVVGPVPL	0.0401
172	31–45	RTGASVVGPVPLPTE	0.0001
Rv0682 (RpsL)
102	18–32	KVKTAALKGSPQRRG	0.0458
103	30–44	RRGVCTRVYTTTPKK	0.0240
Rv1630 (RpsA)
154	431–445	QMEKFAAAEAEAANA	0.0001
Rv3458c (RpsD)
162	1–15	MARYTGPATRKSRRL	0.0018

^aPeptides identified in screens for immunogenic epitopes (Fig. 2 and 3) are listed with their locus tag (name), library identification, peptide number, residue location in the intact protein, amino acid sequence, and statistical significance in the screen conducted. Proteins identified as being immunogenic using the approach illustrated in Fig. 1 are highlighted in boldface type.