FIG 4.
RpoS expression is regulated by cFP via vHUαβ. (A) The effect of cFP on the expression levels of clpX, rssB, dksA, hfq, hns, and vhuAB was assessed by qRT-PCR. RNA was isolated from wild-type V. vulnificus MO6-24/O cultured in AB broth and treated with 0 mM, 1 mM cFP, and 5 mM cFP at early stationary phase (A600, ∼0.2). (B) Expression levels of RpoS as measured by qRT-PCR. RNA samples were obtained from wild-type (pRK415), ΔvhuAB(pRK415), and ΔvhuAB(pRK-vhuAB) cultured in AB broth at early stationary phase (A600, ∼0.2). All the RNA levels were quantified using the comparative threshold cycle (ΔΔCT) method, and RNA fold change values were normalized to the value for MO6-24/O without cFP (*, P < 0.05; NS, not significant).